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Detection of Pt derivatives using ICP mass spectrometry
Zelinová, Karin ; Hložková, Michaela (referee) ; Vašinová Galiová, Michaela (advisor)
This Bachelor´s thesis deals with the monitoring of chemotherapeutic penetration into tumor cells. Due to the toxicity of drugs, targeting them is absolutely essential from the point of view of minimizing the interference with healthy tissue of the patient. In order to achieve the best possible targeting, it is necessary to monitor the penetration of chemotherapeutics into cells. The subject of study was platinum-based drugs therefore the ICP-MS method was chosen to analyse the drug content in cells, because it is suitable for fast and reliable detection of trace amounts of elements. The theoretical part of the Bachelor´s thesis focuses on the description of ICP-MS, as a method, which was chosen for the detection of platinum derivates. It also summarizes the use of platinum-based drugs in cancer therapy. The practical part of the thesis deals with the analysis of cells exposed to platinum-based chemotherapeutics. Detection and quantification of platinum in the cells were determined by both SN-ICP-MS and LA-ICP-MS. To verify the results, the analysis of the solution was also performed by the AAS method. The results show, that the drug was most readily taken up by A2780 cells. It was also shown that cisplatin was the most accumulated drug.
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The study of metallocene anticancer therapeutics-cells interaction
Bilavčíková, Kristýna ; Mravcová, Ludmila (referee) ; Vašinová Galiová, Michaela (advisor)
There were almost 600 thousand patients in Czech Republic until 2017 and the number keeps rising. Chemotherapeutics used today have lots of undesirable side effects, so scientists around the world are trying hard to find more specific and less cytotoxic drugs. In this bachelor’s thesis, potential drugs based on metallocene with a central atom of Ru, Hf or Zr were studied, always in three different drugs for each element. The permeability and distribution of these drugs into lung cancer cell lines were investigated. These drugs were detected by analysis of solutions and laser ablation with an ICP mass spectrometer. Based on the experiment, the greatest potential was found for the ruthenium-based drug 133, which had the highest permeability and was able to get into the cells in the highest volume.
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Detection of Pt derivatives using ICP mass spectrometry
Zelinová, Karin ; Hložková, Michaela (referee) ; Vašinová Galiová, Michaela (advisor)
This Bachelor´s thesis deals with the monitoring of chemotherapeutic penetration into tumor cells. Due to the toxicity of drugs, targeting them is absolutely essential from the point of view of minimizing the interference with healthy tissue of the patient. In order to achieve the best possible targeting, it is necessary to monitor the penetration of chemotherapeutics into cells. The subject of study was platinum-based drugs therefore the ICP-MS method was chosen to analyse the drug content in cells, because it is suitable for fast and reliable detection of trace amounts of elements. The theoretical part of the Bachelor´s thesis focuses on the description of ICP-MS, as a method, which was chosen for the detection of platinum derivates. It also summarizes the use of platinum-based drugs in cancer therapy. The practical part of the thesis deals with the analysis of cells exposed to platinum-based chemotherapeutics. Detection and quantification of platinum in the cells were determined by both SN-ICP-MS and LA-ICP-MS. To verify the results, the analysis of the solution was also performed by the AAS method. The results show, that the drug was most readily taken up by A2780 cells. It was also shown that cisplatin was the most accumulated drug.
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|
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The study of metallocene anticancer therapeutics-cells interaction
Bilavčíková, Kristýna ; Mravcová, Ludmila (referee) ; Vašinová Galiová, Michaela (advisor)
There were almost 600 thousand patients in Czech Republic until 2017 and the number keeps rising. Chemotherapeutics used today have lots of undesirable side effects, so scientists around the world are trying hard to find more specific and less cytotoxic drugs. In this bachelor’s thesis, potential drugs based on metallocene with a central atom of Ru, Hf or Zr were studied, always in three different drugs for each element. The permeability and distribution of these drugs into lung cancer cell lines were investigated. These drugs were detected by analysis of solutions and laser ablation with an ICP mass spectrometer. Based on the experiment, the greatest potential was found for the ruthenium-based drug 133, which had the highest permeability and was able to get into the cells in the highest volume.
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